889 Differences in the 3D nuclear architecture of different epidermal cell sub-populations

Our study aims at characterizing 3D nuclear architecture of distinct sub-populations epidermal cells. We have performed FISH & RASER-FISH, combined with immunofluorescence and analysis widefield SIM super-resolution on cryosections cultured Organization the nucleus significantly differs between cell types during differentiation. Previous studies showed that heratinocyte(KC) differentiation is associated changes in organization. They included the: decrease volume lowering abundance transcriptionally active chromatin, nucleoli numbers their internalization increase pericentromeric heterochromatic clusters(PCs) number. Those affect chromatin organization likely play important role control execution gene expression programmes. analyzed subpopulations keratinocytes basal layer: non-cycling, G1, S G2 phase cells as well Langerhans, merkel T DNA density together IF differences distribution amount heterochromatin those Cycling KCs had more clusters. Langerhans Merkel distributed next to compare T-cells. Results presented show cell-types epidermis differs. data give new insight into biology stresses importance appreciation heterogeneity design research projects.